Regulatory
pcsiD

Part:BBa_K3790009:Design

Designed by: Chongwen Cao   Group: iGEM21_Fudan   (2021-10-01)


The simplest form of csiD promoter, a weak σS promoter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 20
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 20
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 20
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The following primers were used for constructing this part. 

F_A1csiD1_1035	5-TTTTCAGAAATCTAGATATTTTTAGATTAACTAGAGAAAGAGGAGAAATACTAGATGC
R_A1csiD1_1907	5-AATATCTAGATTTCTGAAAAATGCGCACACTCCCACTCCACCTGAACC
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